Abstract:
The current trend in the biopharmaceutical market has boosted the development and production of biological drugs with high efficacy and fidelity for receptor binding. While high-resolution structural insights into binding epitopes of the receptor are indispensable for better therapeutic design, it is tedious and costly. In this work, we develop a protocol by integrating two well-known nmr-based solution-state methods. Saturation transfer double-difference with methyl-trosy (stdd-methyl trosy nmr) was used to probe methyl binding epitopes of the ligand in a label-free environment. This study was carried out with human insulin as a model peptide drug, with the insulin growth factor receptor (igfr), which is an off-target receptor for insulin. Methyl epitopes identified from stdd-methyl trosy nmr spectroscopy were validated through the haddock platform to generate a drug-receptor model. Since this method can be applied at natural abundance, it has the potential to screen a large set of peptide-drug interactions for optimum receptor binding. Thus, we propose stdd-methyl trosy nmr spectroscopy as a technique for rapid screening of biologics for the development of optimized biopharmaceutics. © 2022 wiley-vch gmbh.