N-1 and N-2 positional effects in the propagation of 3(10)-type fold in the helical model peptide Boc-(D)Glu-Pro-Ala-Lys-Ala-Leu-Ala-OMe

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N-1 and N-2 positional effects in the propagation of 3(10)-type fold in the helical model peptide Boc-(D)Glu-Pro-Ala-Lys-Ala-Leu-Ala-OMe

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Title: N-1 and N-2 positional effects in the propagation of 3(10)-type fold in the helical model peptide Boc-(D)Glu-Pro-Ala-Lys-Ala-Leu-Ala-OMe
Author: BERI, S; SRIVASTAVA, S; DHANASEKARAN, M; PHADKE, RS; DURANI, S
Abstract: The N-1 (Ala(2)) and N-2 (Gly(3)) positional amino acids in the type II' turn templated 3(10) helix Boc-(D)Glu(1)-Ala(2)-Gly(3)-Lys(4)-NHMe (1) and Boc-(D)Glu(1)-Ala(2)-Gly(3)-Lys(4)-Ala(5)-Leu(6)-OMe (3) are substituted with Pro(2) and Ala(3) to obtain its variants Boc-(D)Glu(1)-Pro(2)-Ala(3)-Lys(4)-NHMe (2) and Boc-(D)Glu(1)-Pro(2)-Ala(3)-Lys(4)-Ala(5)-Leu(6)-Ala(7)-OMe (4). According to NMR evidence, the resultant peptide (4) is also a 3(10)-type helix endlocked by Boc-(D)Glu(1), with a stronger Lys --> Glu salt bridge but a comparatively weaker helical domain, more susceptible to solvent-mediated disordering. The relatively constrained residues of higher N cap and internal positional helix propensities are thus shown actually to weaken the helical domain in our model, because its helix-templating segment distorts and thus its template-assisted propagation as a 310 helix is compromised. The dependence of geometry and stability of an ordered helical structure on effects in its folding initiation and template-assisted propagation are thus shown to be captured in a helical model peptide of unusual structural simplicity. Copyright (C) 2000 , Ltd.
URI: http://dx.doi.org/10.1002/(SICI)1097-458X(200004)38:4<257::AID-MRC627>3.0.CO;2-W
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9657
http://hdl.handle.net/10054/9657
Date: 2000


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