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dc.contributor.authorBLANTON, MPen_US
dc.contributor.authorLALA, AKen_US
dc.contributor.authorCOHEN, JBen_US
dc.date.accessioned2011-07-25T06:30:47Zen_US
dc.date.accessioned2011-12-26T12:50:04Zen_US
dc.date.accessioned2011-12-27T05:36:02Z
dc.date.available2011-07-25T06:30:47Zen_US
dc.date.available2011-12-26T12:50:04Zen_US
dc.date.available2011-12-27T05:36:02Z
dc.date.issued2001en_US
dc.identifier.citationBIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1512(2), 215-224en_US
dc.identifier.issn0005-2736en_US
dc.identifier.urihttp://dx.doi.org/10.1016/S0005-2736(01)00321-2en_US
dc.identifier.urihttp://dspace.library.iitb.ac.in/xmlui/handle/10054/6662en_US
dc.identifier.urihttp://hdl.handle.net/10054/6662
dc.description.abstractTo identify membrane-associated polypeptides present in Torpedo nicotinic acetylcholine receptor (AChR)-rich membranes, we used hydrophobic photolabeling with [H-3]diazofluorene ([H-3]DAF) and 1-azidopyrene (1-AP) to tag the membrane proteins which were then identified by amino-terminal sequence analysis of labeled fragments isolated from proteolytic digests by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by reverse-phase high-performance liquid chromatography. In addition to AChR subunits, identified polypeptides include the 95 kDa alpha -subunit of the (Na++K+)-ATPase, the 89 kDa voltage-gated chloride channel (CLC-0), the 105 kDa SITS-binding protein, and 32 and 34 kDa polypeptides identified as Torpedo homologues of the mitochondrial membrane ATP/ADP carrier protein and the voltage-dependent anion channel (VDAC), respectively. Further, individual amino acids that reacted with [H-3]DAF and therefore likely to be in contact with lipid were identified in the transmembrane segment M3 of the alpha -subunit of the (Na++K+)-ATPase and in a putative transmembrane beta -strand in VDAC. Collectively these results demonstrate that [H-3]DAF/1-AP photolabeling provides an effective method for tagging the membrane-associated segments of polypeptides in a way that makes it easy to isolate the labeled polypeptide or polypeptide fragments by fluorescence and then to identify amino acids at the lipid-protein interface by H-3 release. (C) 2001 .en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.subjectGated Chloride Channelen_US
dc.subjectAmino-Acid-Sequenceen_US
dc.subjectMitochondrial Porinen_US
dc.subjectElectric Organen_US
dc.subjectPostsynaptic Membranesen_US
dc.subjectSkeletal-Muscleen_US
dc.subjectAgrin Receptoren_US
dc.subjectIon-Channelen_US
dc.subjectProteinen_US
dc.subjectPurificationen_US
dc.subject.other[H-3]Diazofluoreneen_US
dc.subject.otherNicotinic Acetylcholine Receptoren_US
dc.subject.other(Na++K+)-Atpaseen_US
dc.subject.otherVoltage-Dependent Anion Channelen_US
dc.subject.otherPhotoaffinityen_US
dc.titleIdentification and characterization of membrane-associated polypeptides in Torpedo nicotinic acetylcholine receptor-rich membranes by hydrophobic photolabelingen_US
dc.typeArticleen_US


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