1-Naphthol 2-hydroxylase from Pseudomonas sp. strain C6: purification, characterization and chemical modification studies

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1-Naphthol 2-hydroxylase from Pseudomonas sp. strain C6: purification, characterization and chemical modification studies

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dc.contributor.author SAH, S en_US
dc.contributor.author PHALE, PS en_US
dc.date.accessioned 2012-06-26T05:23:23Z
dc.date.available 2012-06-26T05:23:23Z
dc.date.issued 2011 en_US
dc.identifier.citation BIODEGRADATION,22(3)517-526 en_US
dc.identifier.issn 0923-9820 en_US
dc.identifier.uri http://dx.doi.org/10.1007/s10532-010-9424-2 en_US
dc.identifier.uri http://dspace.library.iitb.ac.in/jspui/handle/100/13959
dc.description.abstract 1-Naphthol 2-hydroxylase (1-NH) which catalyzes the conversion of 1-naphthol to 1,2-dihydroxynaphthalene was purified to homogeneity from carbaryl-degrading Pseudomonas sp. strain C6. The enzyme was found to be a homodimer with subunit molecular weight of 66 kDa. UV, visible and fluorescence spectral properties, identification of flavin moiety by HPLC as FAD, and reconstitution of apoenzyme by FAD suggest that enzyme is FAD-dependent. 1-NH accepts electron from NADH as well as NADPH. Besides 1-naphthol (K (m), 9.1 mu M), the enzyme also accepts 5-amino 1-naphthol (K (m), 6.4 mu M) and 4-chloro 1-naphthol (K (m), 2.3 mu M) as substrates. Enzyme showed substrate inhibition phenomenon at high concentration of 1-naphthol (K (i), 283 mu M). Stoichiometric consumption of oxygen and NADH, and biochemical properties suggest that 1-NH belongs to FAD containing external flavomonooxygenase group of oxido-reductase class of enzymes. Based on biochemical and kinetic properties, 1-NH from Pseudomonas sp. strain C6 appears to be different than that reported earlier from Pseudomonas sp. strain C4. Chemical modification and protection by 1-naphthol and NADH suggest that His, Arg, Cys, Tyr and Trp are at or near the active site of 1-NH. en_US
dc.language.iso English en_US
dc.publisher SPRINGER en_US
dc.subject PARA-HYDROXYBENZOATE HYDROXYLASE en_US
dc.subject ADENINE-DINUCLEOTIDE PHOSPHATE en_US
dc.subject PHENOL HYDROXYLASE en_US
dc.subject CRYSTAL-STRUCTURE en_US
dc.subject CATALYTIC CYCLE en_US
dc.subject BINDING-SITE en_US
dc.subject CARBARYL en_US
dc.subject FLAVIN en_US
dc.subject RESIDUE en_US
dc.subject ENZYME en_US
dc.subject.other Carbaryl metabolism en_US
dc.subject.other 1-naphthol 2-hydroxylase en_US
dc.subject.other Flavoenzyme en_US
dc.subject.other Kinetic properties en_US
dc.subject.other Chemical modification en_US
dc.subject.other Active-site residues en_US
dc.title 1-Naphthol 2-hydroxylase from Pseudomonas sp. strain C6: purification, characterization and chemical modification studies en_US
dc.type Article en_US


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