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|Title:||Mechanism of Initiation, Association, and Formation of Amyloid Fibrils Modeled with the N-Terminal Peptide Fragment, IKYLEFIS, of Myoglobin G-Helix|
|Keywords:||Particle Mesh Ewald|
|Publisher:||AMER CHEMICAL SOC|
|Citation:||JOURNAL OF PHYSICAL CHEMISTRY B,121(32)7536-7549|
|Abstract:||Some peptides and proteins undergo self-aggregation under certain conditions, leading to amyloid fibrils formation, which is related to many disease conditions. It is important to understand such amyloid fibrils formation to provide mechanistic detail that governs the process. A predominantly alpha-helical myoglobin has been reported recently to readily form amyloid fibrils at a higher temperature, similar to its G-helix segment. Here, we have investigated the mechanism of amyloid fibrils formation by performing multiple long molecular dynamics simulations (27 mu s) on the N-terminal segment of the G-helix of myoglobin. These simulations resulted in the formation of a single-layered tetrameric beta-sheet with mixed parallel and antiparallel beta-strands and this is the most common event irrespective of many different starting structures. Formation of the single-layered tetrameric beta-sheet takes place following three distinctive pathways. The process of fibril initiation is dependent on temperature. Further, this study provides mechanistic insights into the formation of multilayered fibrilar structure, which could be applicable to a wider variety of peptides or proteins to understand the amyloidogenesis.|
|Appears in Collections:||Article|
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