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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/9780

Title: Lipid peroxidation induced by a novel porphyrin plus light in isolated mitochondria: Possible implications in photodynamic therapy
Authors: CHATTERJEE, SR
SRIVASTAVA, TS
KAMAT, JP
DEVASAGAYAM, TPA
Keywords: photosensitization
biochemistry
microsomes
photofrin
membrane
cancer
tumors
Issue Date: 1997
Publisher: KLUWER ACADEMIC PUBL
Citation: MOLECULAR AND CELLULAR BIOCHEMISTRY, 166(1-2), 25-33
Abstract: With a view to locate porphyrins for use in photodynamic therapy (PDT), the new modality of cancer treatment we have evaluated the ability of a novel water soluble porphyrin meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin (T4CPP) to induce damage to mitochondria during photosensitization. T4CPP, when exposed to visible light, induced lipid peroxidation in rat liver mitochondria as assessed by the formation of thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and lipid hydroperoxides (LOOH). The effect on mitochondrial function was assessed by estimating the activity of succinate dehydrogenase (SDH). The peroxidation induced was observed to be time- and concentration- dependent. Analysis of product formation and selective inhibition by scavengers of reactive oxygen species showed that the oxidative damage observed was mainly due to singlet oxygen (O-1(2)) and partly due to other reactive species. T4CPP plus light also caused significant lipid peroxidation in Sarcoma 180 ascites tumour mitochondria. Our studies indicate that T4CPP has the potential to photoinduce damage in hepatic and ascites mitochondria, a crucial site of damage in PDT.
URI: http://dx.doi.org/10.1023/A:1006840714583
http://dspace.library.iitb.ac.in/xmlui/handle/10054/9780
http://hdl.handle.net/10054/9780
ISSN: 0300-8177
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