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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/898

Title: An integrated mechanistic model for transcription-coupled nucleotide excision repair
Authors: PATEL, SHWETAL
VENKATESH, KV
EDWARDS, JEREMY S
Keywords: cockayne syndrome
in silico biology
hypothesis
uvrabcd
Issue Date: 2004
Publisher: Elsevier
Citation: DNA Repair 3(3), 343-348
Abstract: Preferential repair of the transcribed strand of active genes is usually attributed to a coupling protein that dislodges RNA polymerase stalled at a damage site and recruits repair enzymes. Experimental observations of the effect of transcription on preferential repair in Escherichia coli are contradictory and inexplicable by this model. In this study, it is argued that the multiple conformations displayed by a stalled RNA polymerase result in two sub-pathways for repair: Mfd coupled and direct. Together with the fact that RNA polymerase recruits the repair enzymes in a promoter dependent manner, an integrated mechanistic model is proposed that is capable of explaining the effect of transcription on preferential repair reported in literature. The quantitative behavior of the model is illustrated by describing the various reactions using a biochemical network. The implications of the model on the mechanism for transcription-coupled repair in higher organisms are briefly discussed.
URI: http://dx.doi.org/10.1016/j.dnarep.2003.11.010
http://hdl.handle.net/10054/898
http://dspace.library.iitb.ac.in/xmlui/handle/10054/898
ISSN: 1568-7864
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