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|Title:||A novel steroidal spin label for membrane structure studies: synthesis and applications|
|Publisher:||ELSEVIER SCIENCE INC|
|Citation:||STEROIDS, 64(12), 849-855|
|Abstract:||2,2,6,6-Tetramethyl piperidine-N-oxyl nitroxyls are known to partition between aqueous and lipid phases, thus serving as probes to study membrane dynamics. The synthesis of a novel steroidal spin label, 3 alpha-hydroxycholan-24-yl-(2 ",2 ",6 ",6 "-tetramethyl-N-oxyl)piperidyl butan-1',4'-dioate, containing 2,2,6,6-tetramethylpiperidine-N-oxyl moiety covalently bonded to the side chain in 3,24-caprostan-diol has been described. The localization of this spin label in model biomembranes has been studied by using electron spin resonance, differential scanning calorimetry, and H-1 and P-31 NMR spectroscopic techniques. Its applicability in studying the phase transition properties of model membrane L-alpha-dipalmitoyl phosphatidyl choline in the presence and absence of drugs has been described by using electron spin resonance. The label has also been used to study the permeability of epinephrine into membrane. The results have shown the applicability of the spin label as a potential spin probe in the study of biomembranes. (C) 1999 Elsevier Science Inc. .|
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