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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/6907

Title: Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells
Authors: KUNWAR, A
BARIK, A
MISHRA, B
RATHINASAMY, K
PANDEY, R
PRIYADARSINI, KI
Keywords: serum-albumin
fluorescence
microscopy
mechanism
adhesion
binding
protein
cancer
Issue Date: 2008
Publisher: ELSEVIER SCIENCE BV
Citation: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1780(4), 673-679
Abstract: Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluorescence intensity of curcumin were significantly higher in tumor cells compared to the normal cells. A linear dependency on the uptake was observed with treatment concentration of curcumin. Using laser confocal microscopy, intracellular localization of curcumin was monitored and the results indicated that curcumin is located both in the cell membrane and the nucleus. Sub-cellular fractionation of curcumin-loaded MCF7 cells supported the differential distribution of curcumin in membrane, cytoplasm and nuclear compartments of cell with maximum localization in the membrane. Cytotoxicity studies in different cell lines indicated that the toxicity of curcumin increased with increasing uptake. (C) 2007
URI: http://dx.doi.org/10.1016/j.bbagen.2007.11.016
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6907
http://hdl.handle.net/10054/6907
ISSN: 0304-4165
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