DSpace
 

DSpace at IIT Bombay >
IITB Publications >
Article >

Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/6426

Title: Characterization of silanization and antibody immobilization on spin-on glass (SOG) surface
Authors: NAGARE, GD
MUKHERJI, S
Keywords: silicon
silanes
afm
Issue Date: 2009
Publisher: ELSEVIER SCIENCE BV
Citation: APPLIED SURFACE SCIENCE, 255(6), 3696-3700
Abstract: Most embedded optical waveguide biosensors require low dielectric optical material. The prerequisites for such applications include the selection of an appropriate material and effective bio-functionalization. Spin-on glass (SOG) is one of the preferred polymers that can be easily deposited. Further, the chemical properties of the polymer make it amenable for bio-functionalization. This paper reports a detailed study of a protocol for immobilization of antibodies on SOG for possible immunobiosensor applications. The effect of silanization at different pH values on the number of free and usable amine sites was quantified by fluorimetry. Contact angle measurements, ellipsometry, AFM, and fluorescence microscopy was used for assessing the surface properties at various stages of preparation, functionalization and biomolecule immobilization. (C) 2008
URI: http://dx.doi.org/10.1016/j.apsusc.2008.10.033
http://dspace.library.iitb.ac.in/xmlui/handle/10054/6426
http://hdl.handle.net/10054/6426
ISSN: 0169-4332
Appears in Collections:Article

Files in This Item:

There are no files associated with this item.

View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback