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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/5914

Title: Microtubular conductometric biosensor for ethanol detection
Authors: AJAY, AK
SRIVASTAVA, DN
Keywords: alcohol-dehydrogenase
sensor
polyaniline
films
nad+
Issue Date: 2007
Publisher: ELSEVIER ADVANCED TECHNOLOGY
Citation: BIOSENSORS & BIOELECTRONICS, 23(2), 281-284
Abstract: A conductometric sensor using microtubules of polyaniline as transducer cum immobilization matrix is reported, capable of detecting ethanol in liquid phase. Enzyme ADH (alcohol dehydrogenase) and its coenzyme NAD(+) have been used to improve the selectivity of the sensor. The sensor concept is based on the protonation of the polyaniline by the hydrogen ion produced in the enzyme-catalyzed reaction, leading to changes in the electrical conductance of the polyaniline. The sensor works well on the physiological pH, can detect ethanol as low as 0.02% (v/v) (0.092 M) and has a linear trend at par healthcare guidelines. The sensor responses were measured in various permutation and combination of enzyme and coenzyme concentrations and site of immobilization. The sensor shows minor interference with other functional groups and alcohols. The possible causes for such interference have been discussed. (C) 2007
URI: http://dx.doi.org/10.1016/j.bios.2007.04.019
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5914
http://hdl.handle.net/10054/5914
ISSN: 0956-5663
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