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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/5531

Title: DETERMINATION OF TOTAL PROTON RELEASE IN PURPLE MEMBRANE SUSPENSION BY UMBELLIFERONE FLUORESCENCE QUENCHING TECHNIQUE
Authors: SONAR, S
SINGH, AK
Keywords: halobacterium-halobium
photochemical cycle
bacteriorhodopsin
fragments
Issue Date: 1992
Publisher: COUNCIL SCIENTIFIC INDUSTRIAL RESEARCH
Citation: INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS, 29(3), 251-254
Abstract: A technique for determining total proton release from purple membrane suspension under steady illumination has been described. Illuminated purple membrane is found to quench the fluorescence life-time of umbelliferone indicating the release of protons in the medium. Besides the "stoichiometric" release of protons from bacteriorhodopsin, there seems to be release of protons from sources other than protonated retinylidene Schiff base moiety also.
URI: http://dspace.library.iitb.ac.in/xmlui/handle/10054/5531
http://hdl.handle.net/10054/5531
ISSN: 0301-1208
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