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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/5169

Title: Metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates Alcaligenes sp strain PPH and Pseudomonas sp strain PPD
Authors: DEVERYSHETTY, J
SUVEKBALA, V
VARADAMSHETTY, G
PHALE, PS
Keywords: p-hydroxybenzoate hydroxylase
flavin adenine dinucleotide
salicylate hydroxylase
pathway
monooxygenase
purification
catechol
putida
u2
biodegradation
Issue Date: 2007
Publisher: BLACKWELL PUBLISHING
Citation: FEMS MICROBIOLOGY LETTERS, 268(1), 59-66
Abstract: Pseudomonas sp. strain PPD and Alcaligenes sp. strain PPH isolated from soil by enrichment culture technique utilize 2-, 3- and 4-hydroxybenzoates as the sole source of carbon and energy. The degradation pathways were elucidated by performing whole-cell O-2 uptake, enzyme activity and induction studies. Depending on the mixture of carbon source and the preculture condition, strain PPH was found to degrade 2-hydroxybenzoate either via the catechol or gentisate route and has both salicylate 1-hydroxylase and salicylate 5-hydroxylase. Strain PPD utilizes 2-hydroxybenzoate via gentisate. Both strains degrade 3- and 4-hydroxybenzoate via gentisate and protocatechuate, respectively. Enzymes were induced by respective hydroxybenzoate. Growth pattern, O-2 uptake and enzyme activity profiles on the mixture of three hydroxybenzoates as a carbon source suggest coutilization by both strains. When 3- or 4-hydroxybenzoate grown culture was used as an inoculum, strain PPH failed to utilize 2-hydroxybenzoate via catechol, indicating the modulation of the metabolic pathways, thus generating metabolic diversity.
URI: http://dx.doi.org/10.1111/j.1574-6968.2006.00561.x
http://dspace.library.iitb.ac.in/xmlui/handle/10054/5169
http://hdl.handle.net/10054/5169
ISSN: 0378-1097
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