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|Title: ||Metabolism of 2-, 3- and 4-hydroxybenzoates by soil isolates Alcaligenes sp strain PPH and Pseudomonas sp strain PPD|
|Authors: ||DEVERYSHETTY, J|
|Keywords: ||p-hydroxybenzoate hydroxylase|
flavin adenine dinucleotide
|Issue Date: ||2007|
|Publisher: ||BLACKWELL PUBLISHING|
|Citation: ||FEMS MICROBIOLOGY LETTERS, 268(1), 59-66|
|Abstract: ||Pseudomonas sp. strain PPD and Alcaligenes sp. strain PPH isolated from soil by enrichment culture technique utilize 2-, 3- and 4-hydroxybenzoates as the sole source of carbon and energy. The degradation pathways were elucidated by performing whole-cell O-2 uptake, enzyme activity and induction studies. Depending on the mixture of carbon source and the preculture condition, strain PPH was found to degrade 2-hydroxybenzoate either via the catechol or gentisate route and has both salicylate 1-hydroxylase and salicylate 5-hydroxylase. Strain PPD utilizes 2-hydroxybenzoate via gentisate. Both strains degrade 3- and 4-hydroxybenzoate via gentisate and protocatechuate, respectively. Enzymes were induced by respective hydroxybenzoate. Growth pattern, O-2 uptake and enzyme activity profiles on the mixture of three hydroxybenzoates as a carbon source suggest coutilization by both strains. When 3- or 4-hydroxybenzoate grown culture was used as an inoculum, strain PPH failed to utilize 2-hydroxybenzoate via catechol, indicating the modulation of the metabolic pathways, thus generating metabolic diversity.|
|Appears in Collections:||Article|
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