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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/4635

Title: Modulation of glucose transport causes preferential utilization of aromatic compounds in pseudomonas putida CSV86
Authors: BASU, A
SHRIVASTAVA, R
BASU, B
APTE, SK
PHALE, PS
Keywords: carbon catabolite repression
cyclic adenosine-3',5'-monophosphate levels
camp receptor protein
adenylate-cyclase
escherichia-coli
binding-protein
outer-membrane
aeruginosa
metabolism
pathway
Issue Date: 2007
Publisher: AMER SOC MICROBIOLOGY
Citation: JOURNAL OF BACTERIOLOGY, 189(21), 7556-7562
Abstract: Pseudomonas putida CSV86 utilizes aromatic compounds in preference to glucose and coutilizes aromatics and organic acids. Protein analysis of cells grown on different carbon sources, either alone or in combination, revealed that a 43-kDa periplasmic-space protein was induced by glucose and repressed by aromatics and succinate. Two-dimensional gel electrophoresis and liquid chromatography-tandem mass spectrometry analysis identified this protein as closely resembling the sugar ABC transporter of Pseudomonas putida KT2440. A partially purified 43-kDa protein showed glucose binding activity and was specific for glucose. The results demonstrate that the aromatic- and organic acid-mediated repression of a periplasmic-space glucose binding protein and consequent inhibition of glucose transport are responsible for this strain's ability to utilize aromatics and organic acids in preference to glucose.
URI: http://dx.doi.org/10.1128/JB.01235-07
http://dspace.library.iitb.ac.in/xmlui/handle/10054/4635
http://hdl.handle.net/10054/4635
ISSN: 0021-9193
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