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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/4634

Title: Metabolism of carbaryl via 1,2-dihydroxynaphthalene by soil isolates Pseudomonas dp. strains C4, C5, and C6
Authors: SWETHA, VP
PHALE, PS
Keywords: biochemical-characterization
garden soil
purification
1-naphthol
hydrolysis
hydrolase
biodegradation
degradation
toxicity
rc100
Issue Date: 2005
Publisher: AMER SOC MICROBIOLOGY
Citation: APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(10), 5951-5956
Abstract: Pseudomonas sp. strains C4, C5, and C6 utilize carbaryl as the sole source of carbon and energy. Identification of 1-naphthol, salicylate, and gentisate in the spent media; whole-cell 02 uptake on I-naphthol, 1,2-dihydroxynaphthalene, salicylaidehyde, salicylate, and gentisate; and detection of key enzymes, viz, carbaryl hydrolase, 1-naphthol hydroxylase, 1,2-dihydroxynaphthalene dioxygenase, and gentisate dioxygenase, in the cell extract suggest that carbaryl is metabolized via 1-naphthol, 1,2-dihydroxynaphthalene, and gentisate. Here, we demonstrate I-naphthol hydroxylase and 1,2-dihydroxynaphthalene dioxygenase activities in the cell extracts of carbaryl-grown cells. I-Naphthol hydroxylase is present in the membrane-free cytosolic fraction, requires NAD(P)H and flavin adenine dinucleotide, and has optimum activity in the pH range 7.5 to 8.0. Carbaryl-degrading enzymes are inducible, and maximum induction was observed with carbaryl. Based on these results, the proposed metabolic pathway is carbaryl -> 1-naphthol -> 1,2-dihydroxynaphthalene salicylaldehyde -> salicylate -> gentisate -> maleylpyruvate.
URI: http://dx.doi.org/10.1128/AEM.71.10.5951-5956.2005
http://dspace.library.iitb.ac.in/xmlui/handle/10054/4634
http://hdl.handle.net/10054/4634
ISSN: 0099-2240
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