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|Title:||Metabolism of carbaryl via 1,2-dihydroxynaphthalene by soil isolates Pseudomonas dp. strains C4, C5, and C6|
|Publisher:||AMER SOC MICROBIOLOGY|
|Citation:||APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(10), 5951-5956|
|Abstract:||Pseudomonas sp. strains C4, C5, and C6 utilize carbaryl as the sole source of carbon and energy. Identification of 1-naphthol, salicylate, and gentisate in the spent media; whole-cell 02 uptake on I-naphthol, 1,2-dihydroxynaphthalene, salicylaidehyde, salicylate, and gentisate; and detection of key enzymes, viz, carbaryl hydrolase, 1-naphthol hydroxylase, 1,2-dihydroxynaphthalene dioxygenase, and gentisate dioxygenase, in the cell extract suggest that carbaryl is metabolized via 1-naphthol, 1,2-dihydroxynaphthalene, and gentisate. Here, we demonstrate I-naphthol hydroxylase and 1,2-dihydroxynaphthalene dioxygenase activities in the cell extracts of carbaryl-grown cells. I-Naphthol hydroxylase is present in the membrane-free cytosolic fraction, requires NAD(P)H and flavin adenine dinucleotide, and has optimum activity in the pH range 7.5 to 8.0. Carbaryl-degrading enzymes are inducible, and maximum induction was observed with carbaryl. Based on these results, the proposed metabolic pathway is carbaryl -> 1-naphthol -> 1,2-dihydroxynaphthalene salicylaldehyde -> salicylate -> gentisate -> maleylpyruvate.|
|Appears in Collections:||Article|
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