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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/3469

Title: Photodynamic effects induced by meso-tetrakis[4(carboxymethyleneoxy)phenyl] porphyrin using rat hepatic microsomes as model membranes
Authors: CHATTERJEE, SR
MURUGESAN, S
KAMAT, JP
SHETTY, SJ
SRIVASTAVA, TS
NORONHA, OPD
SAMUEL, AM
DEVASAGAYAM, TPA
Keywords: singlet molecular-oxygen
lipid-peroxidation
strand breaks
therapy
photofrin
hematoporphyrin
sequestration
mitochondria
cancer
light
Issue Date: 1997
Publisher: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
Citation: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 339(1), 242-249
Abstract: Porphyrins, in combination with light, offer an alternate approach to the treatment of cancer, in the form of photodynamic therapy (PDT). With a view to locate new porphyrins for use in PDT, we evaluated the ability of a novel water-soluble porphyrin, meso-tetrakis[4-(carboxymethyleneoxy)phenyl]porphyrin (T4CPP) to induce photodamage in membranes, using rat hepatic microsomes as a model system. Hepatic microsomes treated with T4CPP and exposed to visible light showed significant lipid peroxidation, as assessed by the formation of conjugated dienes, lipid hydroperoxides, and thiobarbituric acid-reactive substances. The peroxidation induced was both time- and concentration-dependent. T4CPP plus light also resulted in the destruction of the microsomal enzymes adenosine triphosphatase and glucose-6-phosphatase. Analysis of the products of peroxidation and selective inhibition by specific inhibitors showed that the oxidative damage induced was mainly due to singlet oxygen and partly due to hydroxyl radical. The porphyrin T4CPP was efficiently labeled with Tc-99m. When this Tc-99m-labeled porphyrin was injected into a mammary-tumor-bearing rat, it accumulated in the tumor. Our studies suggest that T4CPP, due to its potential to localize in tumors and to induce membrane damage as exemplified by alteration in rat liver microsomes, may have possible applications in this new modality of cancer treatment. (C) 1997
URI: http://dx.doi.org/10.1006/abbi.1996.9846
http://dspace.library.iitb.ac.in/xmlui/handle/10054/3469
http://hdl.handle.net/10054/3469
ISSN: 0003-9861
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