Please use this identifier to cite or link to this item:
|Title:||A shorter peptide model from staphylococcal nuclease for the folding-unfolding equilibrium of a beta-hairpin shows that unfolded state has significant contribution from compact conformational states|
Headpiece Helical Subdomain
Particle Mesh Ewald
|Publisher:||ACADEMIC PRESS INC ELSEVIER SCIENCE|
|Citation:||JOURNAL OF STRUCTURAL BIOLOGY, 164(1), 60-74|
|Abstract:||It is important to understand the conformational features of the unfolded state in equilibrium with folded state under physiological. In this paper, we consider a short peptide model LMYKGQPM from staphylococcal nuclease to model the conformational equilibrium between a hairpin conformation and its unfolded state using molecular dynamics simulation under NVT conditions at 300 K Using GROMOS96 force held. The free energy landscape has overall funnel-like shape with hairpin conformations sampling the minima. The "unfolded" state has a higher free energy of similar to 12 kJ/mol with respect to native hairpin mininium and occupies a plateau region. We find that the unfolded state has significant contributions from compact conformations. Many of these conformations have hairpin-like topology. Further, these compact conformational forms are stabilized by hydrophobic interactions. Conversion between native and non-native hairpins occurs via unfolded states. Frequent conversions between folded and Unfolded hairpins are observed with single exponential kinetics. We compare our results With the emerging picture of unfolded state from both experimental and theoretical studies. (c) 2008|
|Appears in Collections:||Article|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.