DSpace
 

DSpace at IIT Bombay >
IITB Publications >
Article >

Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/12835

Title: Labeling TiO(2) Nanoparticles with Dyes for Optical Fluorescence Microscopy and Determination of TiO(2)-DNA Nanoconjugate Stability
Authors: THURN, KT
PAUNESKU, T
WU, AG
BROWN, EMB
LAI, B
VOGT, S
MASER, J
ASLAM, M
DRAVID, V
BERGAN, R
WOLOSCHAK, GE
Keywords: titanium-dioxide nanoparticles
alizarin red-s
in-vitro
antisense oligonucleotides
intracellular trafficking
charge separation
binding proteins
mammalian-cells
ultrafine tio2
particles
Issue Date: 2009
Publisher: WILEY-V C H VERLAG GMBH
Citation: SMALL, 5(11), 1318-1325
Abstract: Visualization of nanoparticles without intrinsic optical fluorescence properties is a significant problem when performing intracellular studies. Such is the case with titanium dioxide (TiO(2)) nanoparticles. These nanoparticles, when electronically linked to single-stranded DNA oligonucleotides, have been proposed to be used both as gene knockout devices and as possible tumor imaging agents. By interacting with complementary target sequences in living cells, these photoinducible TiO(2)-DNA nanoconjugates have the potential to cleave intracellular genomic DNA in a sequence specific and inducible manner. The nanoconjugates also become detectable by magnetic resonance imaging with the addition of gadolinium Gd(III) contrast agents. Herein two approaches for labeling TiO(2) nanoparticles and TiO(2)-DNA nanoconjugates with optically fluorescent agents are described. This permits direct quantification of fluorescently labeled TiO(2) nanoparticle uptake in a large population of living cells (>10(4) cells). X-ray fluorescence microscopy (XFM) is combined with fluorescent microscopy to determine the relative intracellular stability of the nanoconjugates and used to quantify intracellular nanoparticles. Imaging the DNA component of the TiO(2)-DNA nanoconjugate by fluorescent confocal microscopy within the same cell shows an overlap with the titanium signal as mapped by XFM. This strongly implies the intracellular integrity of the TiO(2)-DNA nanoconjugates in malignant cells.
URI: http://dx.doi.org/10.1002/smll.200801458
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12835
http://hdl.handle.net/10054/12835
ISSN: 1613-6810
Appears in Collections:Article

Files in This Item:

There are no files associated with this item.

View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback