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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/12300

Title: Metabolism of benzyl alcohol via catechol ortho-pathway in methylnaphthalene-degrading Pseudomonas putida CSV86
Authors: BASU, A
DIXIT, SS
PHALE, PS
Keywords: meta-cleavage pathway
tol plasmid pww0
xylene monooxygenase
benzaldehyde dehydrogenase
cunninghamella-elegans
oxidative-metabolism
toluene dioxygenase
aromatic-compounds
arvilla mt-2
crude-oil
Issue Date: 2003
Publisher: SPRINGER-VERLAG
Citation: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 62(5-6), 579-585
Abstract: Pseudomonas putida CSV86 metabolizes 1- and 2-methylnaphthalene through distinct catabolic and detoxification pathways. In spite of the similarity in the steps involved in the methylnaphthalene detoxification and the toluene side-chain hydroxylation pathways, the strain failed to utilize toluene or xylenes. However, it could grow on benzyl alcohol, 2- and 4-hydroxybenzyl alcohol. Metabolic studies suggest that the benzyl alcohol metabolism proceeds via the benzaldehyde, benzoate, and catechol ortho-cleavage pathway, in contrast to the well established catechol meta-cleavage pathway. Carbon source-dependent enzyme activity studies suggest that the degradation of aromatic alcohol involves two regulons. Aromatic alcohol induces the upper regulon, which codes for aromatic alcohol- and aromatic aldehyde-dehydrogenase and converts alcohol into acid. The aromatic acid so generated induces the specific lower regulon and is metabolized via either the ortho- or the meta-cleavage pathway. CSV86 cells transform 1- and 2-methylnaphthalene to 1- and 2-hydroxymethyl naphthalene, which are further converted to the respective naphthoic acids due to the basal level expression and broad substrate specificity of the upper regulon enzymes.
URI: http://dx.doi.org/10.1007/s00253-003-1305-8
http://dspace.library.iitb.ac.in/xmlui/handle/10054/12300
http://hdl.handle.net/10054/12300
ISSN: 0175-7598
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