DSpace
 

DSpace at IIT Bombay >
IITB Publications >
Article >

Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/1216

Title: Silanization and antibody immobilization on SU-8
Authors: JOSHI, M
PINTO, RICHARD
RAMGOPAL RAO, V
MUKHERJI, SOUMYA
Keywords: antibodies
hydrolysis
silanes
sensors
microelectromechanical devices
Issue Date: 2007
Publisher: Elsevier
Citation: Applied Surface Science 253(6), 3127-3132
Abstract: SU-8, an epoxy based negative photoresist, has emerged as a structural material for microfabricated sensors due to its attractive mechanical properties like low Young's modulus and chemical properties like inertness to various chemicals used in microfabrication. It can be used to fabricate MEMS structures of high aspect ratio. However, the use of SU-8 in BioMEMS application has been limited by the fact that immobilization of biomolecules on SU-8 surfaces has not been reported. In this study, the epoxy groups on the SU-8 surface were hydrolyzed in the presence of sulphochromic solution. Following this, the surface was treated with [3-(2-aminoethyl) aminopropyl]-trimethoxysilane (AEAPS). The silanized SU-8 surface was used to incubate human immunoglobulin (HIgG). The immobilization of HIgG was proved by allowing FITC tagged goat anti-human IgG to react with HIgG. This process of antibody immobilization was used to immobilize HIgG on microfabricated SU-8 cantilevers.
URI: 10.1016/j.apsusc.2006.07.017
http://hdl.handle.net/10054/1216
http://dspace.library.iitb.ac.in/xmlui/handle/10054/1216
ISSN: 0169-4332
Appears in Collections:Article

Files in This Item:

File Description SizeFormat
Soumyo444230.pdf904.37 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback