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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/11578

Title: Curcumin inhibits FtsZ assembly: an attractive mechanism for its antibacterial activity
Authors: RAI, D
SINGH, JK
ROY, N
PANDA, D
Keywords: division protein ftsz
cell-division
bacterial cytokinesis
targeting ftsz
small-molecule
gtp
degradation
stability
discovery
products
Issue Date: 2008
Publisher: PORTLAND PRESS LTD
Citation: BIOCHEMICAL JOURNAL, 410(), 147-155
Abstract: The assembly and stability of FtsZ protofilaments have been shown to play critical roles in bacterial cytokinesis. Recent evidence suggests that FtsZ may be considered as an important antibacterial drug target. Curcumin, a dietary polyphenolic compound, has been shown to have a potent antibacterial activity against a number of pathogenic bacteria including Staphylococcus aureus, Staphylococcus epidermidis and Enterococcus. We found that curcumin induced filamentation in the Bacillus subtilis 168, suggesting that it inhibits bacterial cytokinesis. Further, curcumin strongly inhibited the formation of the cytokinetic Z-ring in B. subtilis 168 without detectably affecting the segregation and organization of the nucleoids. Since the assembly dynamics of FtsZ protofilaments plays a major role in the formation and functioning of the Z-ring, we analysed the effects of curcumin on the assembly of FtsZ protofilaments. Curcumin inhibited the assembly of FtsZ protofilaments and also increased the GTPase activity of FtsZ. Electron microscopic analysis showed that curcumin reduced the bundling of FtsZ protofilaments in vitro. Further, curcumin was found to bind to FtsZ in vitro with a dissociation constant of 7.3 +/- 1.8 mu M and the agent also perturbed the secondary structure of FtsZ. The results indicate that the perturbation of the GTPase activity of FtsZ assembly is lethal to bacteria and suggest that curcumin inhibits bacterial cell proliferation by inhibiting the assembly dynamics of FtsZ in the Z-ring.
URI: http://dx.doi.org/10.1042/BJ20070891
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11578
http://hdl.handle.net/10054/11578
ISSN: 0264-6021
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