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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/11508

Title: Type II phosphatidylinositol 4-kinase beta associates with TCR-CD3 zeta chain in Jurkat cells
Authors: SRIVASTAVA, R
SINHA, RK
SUBRAHMANYAM, G
Keywords: antigen-receptor
protein-tyrosine
phosphoinositide kinases
splenic lymphocytes
signal-transduction
actin cytoskeleton
4-kinase isoforms
t-cells
3-kinase
phosphorylation
Issue Date: 2006
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 43(5), 454-463
Abstract: Phosphatidylinositol lipid signaling cascades are integral part of TCR-CD3 signaling. The mechanisms by which phosphatidylinositol kinases are coupled to TCR-CD3 complex remain elusive. Here we report an association of type II PtdIns 4-kinase with TCR-CD3 zeta chain upon cross-linking. Mapping studies have revealed that the C-terminal ITAM is critical for docking of the enzyme on the zeta chain. The association is shown to be tyrosyl phosphorylation dependent as mutation of Y-151 and Y-142 on the C-terminal ITAM disrupts interaction of the two proteins. Identification of the associated type II PtdIns 4-kinase revealed that the beta isoform of the enzyme interacts with the zeta chain in vivo. (c) 2005
URI: http://dx.doi.org/10.1016/j.molimm.2005.03.009
http://dspace.library.iitb.ac.in/xmlui/handle/10054/11508
http://hdl.handle.net/10054/11508
ISSN: 0161-5890
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