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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10839

Title: Fc epsilon RI cross-linking activates a type II phosphatidylinositol 4-kinase in RBL 2H3 cells
Authors: NAVEEN, B
SHANKAR, BS
SUBRAHMANYAM, G
Keywords: mediated tyrosine phosphorylation
neuronal calcium sensor-1
rat splenic lymphocytes
ige receptor
mast-cells
lipid rafts
phosphoinositide kinases
rbl-2h3 cells
association
actin
Issue Date: 2005
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 42(12), 1541-1549
Abstract: Crosslinking of Fc epsilon RI on rat basophilic leukemia (RBL 2H3) cells leads to an increase in Phosphatidylinositol 4-kinase activity. This increase in Ptdlns 4-kinase activity is strongly correlated with its tyrosyl phosphorylation state. Characterization of the enzyme activity in anti phosphotyrosine immunoprecipitates suggests it as a type II Ptdlns 4-kinase. Membrane cholesterol depletion studies showed a reduction in type II Ptdlns 4-kinase activity suggesting that lipid rafts play an important role in activation of the enzyme. The enzyme activity was inhibited by resveratrol. In situ inhibition of type II Ptdlns 4-kinase activity showed a reduction in beta-hexosaminidase release upon Fc epsilon RI cross-linking. These studies suggest that a type II Ptdlns 4-kinase is an integral component of Fc epsilon RI mediated signal transduction mechanisms. (c) 2005
URI: http://dx.doi.org/10.1016/j.molimm.2004.12.019
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10839
http://hdl.handle.net/10054/10839
ISSN: 0161-5890
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