DSpace
 

DSpace at IIT Bombay >
IITB Publications >
Article >

Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10635

Title: Concanavalin A modulates tyrosine phosphorylation and activation of a type II phosphatidylinositol 4-kinase in rat splenic lymphocytes
Authors: FERNANDIS, AZ
SUBRAHMANYAM, G
Keywords: t-cell activation
antigen receptor
signal-transduction
phosphoinositide kinases
protein
purification
stimulation
turnover
pathways
binding
Issue Date: 1998
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 35(14-15), 935-941
Abstract: Activation of rat splenic lymphocytes by concanavalin A resulted in two-fold increase in PtdIns 4-kinase activity and rapid tyrosine phosphorylation of the enzyme. The activation kinetics showed a strong correlation with tyrosine phosphorylation state of the enzyme. Characterization of the enzyme activity suggests that it is a type II PtdIns 4-kinase. Kinetic analysis of the enzyme reaction showed three-fold decrease in K-m for PtdIns and two-fold increase in V-max in Con A stimulated cells. These results suggest that a type II PtdIns 4-kinase is an integral component of the early signal transduction machinery during T-cell activation by concanavalin A and is actively regulated by protein tyrosine phosphorylation-dephosphorylation. (C) 1998 . .
URI: http://dx.doi.org/10.1016/S0161-5890(98)00078-9
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10635
http://hdl.handle.net/10054/10635
ISSN: 0161-5890
Appears in Collections:Article

Files in This Item:

There are no files associated with this item.

View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback