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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10488

Title: A type II phosphatidylinositol 4-kinase associates with T cell receptor zeta chain in Con A stimulated splenic lymphocytes through tyrosyl phosphorylation-dependent mechanisms
Authors: FERNANDIS, AZ
SRIVASTAVA, R
SINHA, RK
SUBRAHMANYAM, G
Keywords: actin cytoskeleton
phosphoinositide kinases
immunological synapse
signal-transduction
activation
4-kinase
antigen
purification
platelets
3-kinase
Issue Date: 2005
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Citation: MOLECULAR IMMUNOLOGY, 42(5), 561-568
Abstract: T cells show rapid reorganization of cytoskeleton in response to antigenic stimulation. The molecular mechanisms by which TCR-CD3 regulates actin cytoskeleton are not well defined. Here we show that a type II PtdIns 4-kinase associates with cytoskeletal fraction in splenic lymphocytes in response to Con A. Protein tyrosyl phosphorylation of type 11 PtdIns 4-kinase appears to be the mechanism for its association with cytoskeleton. Over-Jay blots suggest that the enzyme binds to TCR-CD3 chain in the cytoskeletal fraction. Anti-TCR-CD3 C antibodies competitively inhibit PtdIns 4-kinase association with TCR-CD3 chain. Immunodepletion of TCR-CD3 zeta decreases PtdIns 4-kinase activity in the cytoskeletal fraction with a concomitant increase in PtdIns 4-kinase activity in anti-TCR-CD3 zeta immunoprecipitates. We propose that the association of type II PtdIns 4-kinase with TCR-CD3 chain may bring the enzyme into close proximity of actin and a possible regulation of actin polymerization through localized production of PtdIns4P and PtdIns(4,5)P-2. (c) 2004
URI: http://dx.doi.org/10.1016/j.molimm.2004.09.016
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10488
http://hdl.handle.net/10054/10488
ISSN: 0161-5890
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