DSpace
 

DSpace at IIT Bombay >
IITB Publications >
Article >

Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10316

Title: Axial resolution limit of a fiber-optic fluorescence probe
Authors: BALAJI, J
GARAI, K
CHAKRABARTI, S
MAITI, S
Keywords: confocal reflectance microscope
correlation spectroscopy
fluctuations
biosensors
system
Issue Date: 2003
Publisher: OPTICAL SOC AMER
Citation: APPLIED OPTICS, 42(19), 3780-3784
Abstract: We examine the limit of spatial resolution achievable when a single optical fiber is used for excitation and collection of fluorescence from a bulk specimen. We calculate the probability of detecting a fluorescent particle as a function of its position relative to the fiber face, using excitation wavelength X, radius a, numerical aperture N.A., and the particle's fluorescence and absorbance spectra. Treating Rhodamine B as a model fluorescent analyte and using appropriate fiber parameters, we show that the maximum axial resolution (defined as the axial distance in a homogenous solution within which 50% of the detected signal originates) achievable is similar to10 mum. We experimentally measured the axial resolution for a 500-muM aqueous solution of Rhodamine B with lambda = 543 nm, a = 1.31 mum, and a N.A. of 0.16 and found good qualitative agreement with the calculation. (C) 2003 Optical Society of America.
URI: http://dx.doi.org/10.1364/AO.42.003780
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10316
http://hdl.handle.net/10054/10316
ISSN: 0003-6935
Appears in Collections:Article

Files in This Item:

There are no files associated with this item.

View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback