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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10228

Title: Enhancement of NAPL bioavailability by induction of cell-surface hydrophobicity in Exiguobacterium aurantiacum and Burkholderia cepacia
Authors: MOHANTY, G
MUKHERJI, S
Keywords: pseudomonas-aeruginosa
crude-oil
microbial uptake
diesel oil
hydrocarbon
bioremediation
lipopolysaccharide
biodegradation
microorganisms
bacteria
Issue Date: 2008
Publisher: NATL INST SCIENCE COMMUNICATION
Citation: INDIAN JOURNAL OF BIOTECHNOLOGY, 7(3), 295-306
Abstract: Induction of cell surface hydrophobicity in bacterial cultures can facilitate the direct interfacial uptake of non-aqueous phase liquids (NAPLs). This study explores bioavailability of NAPLs for Exiguobacterium aurantiacum and Burkholderia cepacia isolated from oil-contaminated soil and sediments. Surface tension measurements and emulsification activity tests did not provide evidence for release of extracellular biosurfactants/bioemulsifiers. Contact angle measurement on cell layers and bacterial adhesion to hydrocarbon (BATH) assay was conducted for determining the cell surface hydrophobicity. While the surfaces of cultures grown on soluble substrate, dextrose, were not hydrophobic, higher water contact angle and greater adherence on n-hexadecane/diesel revealed the highly hydrophobic nature of the cell surfaces for cultures grown on NAPLs (n-hexadecane and diesel), thus providing evidence for induction in cell surface hydrophobicity. Positive results in the carbocyanine assay indicating release of lipopolysaccharides/extracellular polysaccharides was observed over the log growth phase only for the NAPL-grown cells. Transmission electron microscopy (TEM) revealed an abundance of intracellular electron transparent globules within the NAPL-grown cells. Light microscopy and TEM images together revealed differences in cell surface characteristics of E. aurantiacum and B. cepacia, which was also affected by the growth substrate.
URI: http://dspace.library.iitb.ac.in/xmlui/handle/10054/10228
http://hdl.handle.net/10054/10228
ISSN: 0972-5849
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