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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/10054/10042

Title: Fabrication of Mineralized Polymeric Nanofibrous Composites for Bone Graft Materials
Authors: NGIAM, M
LIAO, S
PATIL, AJ
CHENG, ZY
YANG, FY
GUBLER, MJ
RAMAKRISHNA, S
CHAN, CK
Keywords: osteoblast-like cells
nanophase ceramics
in-vitro
extracellular-matrix
tissue regeneration
chitosan scaffolds
osteogenic cells
collagen matrix
hydroxyapatite
growth
Issue Date: 2009
Publisher: MARY ANN LIEBERT INC
Citation: TISSUE ENGINEERING PART A, 15(3), 535-546
Abstract: Poly-L-lactic acid (PLLA) and PLLA/collagen (50% PLLA+50% collagen; PLLA/Col) nanofibers were fabricated using electrospinning. Mineralization of these nanofibers was processed using a modified alternating soaking method. The structural properties and morphologies of mineralized PLLA and PLLA/Col nanofibers were investigated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and contact angle measurements. Human bone-derived osteoblasts were cultured on the materials for up to 1 week to assess the biological properties of the nanofibrous composites. Cell attachment on these nanocomposites was also tested within 1 h of culture at room temperature. The mechanical properties of the cell-nanocomposite constructs were determined using tensile testing. From our results, the bone-like nano-hydroxyapatite (n-HA) was successfully deposited on the PLLA and PLLA/Col nanofibers. We observed that the formation of n-HA on PLLA/Col nanofibers was faster and significantly more uniform than on pure PLLA nanofibers. The n-HA significantly improved the hydrophilicity of PLLA/Col nanofibers. From the results of cell attachment studies, n-HA deposition enhanced the cell capture efficacy at the 20-minute time point for PLLA nanofibers. The E-modulus values for PLLA+n-HA with cells (day 1 and day 4) were significantly higher than for PLLA+n-HA without cells. Based on these observations, we have demonstrated that n-HA deposition on nanofibers is a promising strategy for early cell capture.
URI: http://dx.doi.org/10.1089/ten.tea.2008.0011
http://dspace.library.iitb.ac.in/xmlui/handle/10054/10042
http://hdl.handle.net/10054/10042
ISSN: 1937-3341
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