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Title: Cell-free synthesis-based protein microarrays and their applications
Authors: CHANDRA, H
Keywords: cell-free expression
multiple spotting technique
nucleic acid programmable protein array
protein in situ array
protein microarrays
Issue Date: 2010
Citation: PROTEOMICS,10(4)717-730
Abstract: The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
URI: http://dx.doi.org/10.1002/pmic.200900462
ISSN: 1615-9853
Appears in Collections:Review

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