Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/3007
Title: Cell-free synthesis-based protein microarrays and their applications
Authors: CHANDRA, H
SRIVASTAVA, S
Keywords: Cell-Free Expression
Multiple Spotting Technique
Nucleic Acid Programmable Protein Array
Protein In Situ Array
Protein Microarrays
Issue Date: 2010
Publisher: WILEY-V C H VERLAG GMBH
Citation: PROTEOMICS,10(4)717-730
Abstract: The entire complement of proteins expressed by the genome of an organism under a set of defined conditions represents the proteome. The need for rapid, efficient, and high-throughput technologies to understand the proteome of organisms have resulted in the development of protein microarrays. Traditional, cell-based technologies for the generation of protein arrays involve laborious and protracted procedures, which also pose several other technical problems. These limitations have been suitably overcome by cell-free expression systems that carry out rapid, in situ synthesis of proteins from their corresponding DNA templates. This article provides an overview of commonly used cell-free systems that have been used for the generation of protein microarrays, with special emphasis on the diverse applications of cell-free synthesis-based protein microarrays.
URI: http://dx.doi.org/10.1002/pmic.200900462
http://dspace.library.iitb.ac.in/xmlui/handle/10054/13772
http://hdl.handle.net/100/3007
ISSN: 1615-9853
Appears in Collections:Review

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.