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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/100/2905

Title: A low voltage single cell electroporator with a microfabricated sense-porate aperture
Authors: SARKAR, A
MITRA, B
SHASTRY, A
WADIA, S
MULHERKA, R
LAL, R
Issue Date: 2004
Publisher: IEEE
Citation: MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST,375-378
Abstract: We present a novel flow-type single cell electroporation (SCE) system for low voltage electroporation of biological cells. We have used a microfabricated silicon sense-porate aperture to detect and identify a cell by its impedance and then apply the optimum electric field on it. Incorporation of a fluorescent dye into mouse embryo fibroblast NIH-3T3 cells has been demonstrated with a cell survival rate or viability much higher than conventional macroelectroporators. This paper reports the principle, design and implementation of this system and the experimental results obtained using it.
URI: http://dspace.library.iitb.ac.in/xmlui/handle/10054/16412
http://hdl.handle.net/100/2905
ISBN: 0-7803-8265-X
ISSN: 1084-6999
Appears in Collections:Proceedings papers

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