Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/18027
Title: Zn2+ and Cu2+ induced nanosheets and nanotubes in six different lectins by TEM
Authors: TABBASUM, K
RAO, CP
Keywords: Islet Amyloid Polypeptide
Peptide Nanotubes
Nanocrystal Growth
Protein Crystals
Fibril Formation
Nanostructures
Capabilities
Hexapeptide
Chemistry
Fragments
Issue Date: 2015
Publisher: ROYAL SOC CHEMISTRY
Citation: RSC ADVANCES, 5(22)16828-16836
Abstract: Self assembly of biomolecules is important not only in unravelling the fundamental aspects of biology but also in exhibiting their supramolecular structures wherein the self assembly acts as a basic principle. Therefore, proteins having different side chains and structures with varying binding affinities towards metal ions can certainly be manifested in generating myriad supramolecular structures. In order to demonstrate this, we have chosen several beta-sheet lectins, such as, Horse gram lectin (DBL), Pisum sativum agglutinin (PSA), Wheat germ agglutinin (WGA), Allium sativum agglutinin also known as garlic lectin (ASA) and Concanavalin A (Con A) lectin, in conjunction with Zn2+ and Cu2+ as biologically important ions and showed that these form nanostructures, such as, open-, rolled-and stacked sheets, and tubes. The Zn2+ induces mainly the sheets in the lectins studied so far though some of these are thin and others are thick, while some are folded and others are not. However, Cu2+ induces mainly tubes in ASA and DBL, predominantly sheets in Con A and a mixture of tubes and sheets in PSA and WGA as shown by TEM and supported by SEM. The tubes formed were thick as well as thin, solid as well as hollow in nature, straight, cylindrical as well as twisted and ribbon shaped. Plasmid PBR322 treated nanostructures formed from PSA in the presence of Cu2+ showed the presence of the plasmid in the corresponding tubes and sheets. These nanostructures are unprecedented and could lead to major advances in biomaterials science by providing potential applications.
URI: http://dx.doi.org/10.1039/c5ra00481k
http://dspace.library.iitb.ac.in/jspui/handle/100/18027
ISSN: 2046-2069
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