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|Title:||Coumarine-imino-C2-glucosyl conjugate as receptor for Cu2+ in blood serum milieu, on silica gel sheet and in Hep G2 cells and the characterization of the species of recognition|
|Publisher:||ELSEVIER SCIENCE BV|
|Citation:||ANALYTICA CHIMICA ACTA, 873,80-87|
|Abstract:||A coumarine-imino-C2-glucosyl conjugate (L) was synthesized and characterized. The conjugate L is found to recognize Cu2+ in aqueous HEPES buffer by exhibiting a 95% fluorescence quenching in pH range 7-10 even in the presence of several biologically and ecologically relevant metal ions. Fluorescence on-off behavior has been clearly demonstrated on the basis of the binding variability of Cu2+ to L. The binding has been elicited through the changes observed in fluorescence, absorption, ESI-MS and H-1 NMR titrations. All the other thirteen metal ions studied did not show any change in the fluorescence emission. These ions do not interfere with the recognition of Cu2+ by L. The structural features of [CuL](2) complex in both the isomeric forms were established by DFT computational calculations. The utility of L has been demonstrated by showing its sensitivity toward Cu2+ on a thin layer of silica gel. The L gives sensitive fluorescence signals for Cu2+ even in blood serum and exhibits appropriate fluorescence responses in living cells. (C) 2015 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Article|
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