Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/17103
Title: A dps promoter based expression system for improved solubility of expressed proteins in Escherichia coli
Authors: SETHIA, PP
RAO, KK
NORONHA, SB
Keywords: Escherichia Coli
Recombinant Protein Expression
Dps Promoter
Protein Solubility
Issue Date: 2014
Publisher: KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Citation: BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 19(5)790-797
Abstract: Escherichia coli is widely used for recombinant protein production due to its well established genetic manipulation techniques and cost effectiveness of the associated production processes. Soluble expression of heterologous recombinant proteins constitutes a major problem in the deployment of bacterial expression systems. We have developed a dps promoter based expression system in E. coli for improved solubility of expressed proteins. The resulting expression system was found to be superior to the IPTG inducible T7 promoter based pET expression system for production of soluble beta-galactosidase, tdTomato, and mCherry. The dps promoter based expression system was shown to be functional in most commonly used strains of E. coli without need for prior genetic manipulation of the host genome.
URI: http://dx.doi.org/10.1007/s12257-013-0722-5
http://dspace.library.iitb.ac.in/jspui/handle/100/17103
ISSN: 1226-8372
1976-3816
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