Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/16804
Title: Elementary mode analysis reveals that Clostridium acetobutylicum modulates its metabolic strategy under external stress
Authors: KUMAR, M
SAINI, S
GAYEN, K
Issue Date: 2014
Publisher: ROYAL SOC CHEMISTRY
Citation: MOLECULAR BIOSYSTEMS, 10(8)2090-2105
Abstract: Clostridium acetobutylicum is a strict anaerobe which exhibits two distinct steps in its metabolic network. In the first step, sugars are oxidized to organic acids (acetic and butyric). This is accompanied with growth. The acids produced in the first phase are re-assimilated into solvents (acetone, butanol, and ethanol) in the second phase of metabolism. The two phases are hence called acidogenesis and solventogenesis, respectively. In this work, using Elementary Mode Analysis (EMA), we quantify fluxes through Elementary Modes under different physical and chemical conditions. Our analysis reveals that, in response to external stresses, the organism invokes Elementary Modes which couple acidogenesis and solventogenesis. This coupling leads to the organism exhibiting characteristics of both, acidogenesis and solventogenesis at the same time. Significantly, this coupling was not invoked during any "unstressed" conditions tested in this study. Overall, our work highlights the flexibility in Clostridium acetobutylicum to modulate its metabolism to enhance chances of survival under harsh conditions.
URI: http://dx.doi.org/10.1039/c4mb00126e
http://dspace.library.iitb.ac.in/jspui/handle/100/16804
ISSN: 1742-206X
1742-2051
Appears in Collections:Article

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.