Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/16107
Title: Model based optimization of high cell density cultivation of nitrogen-fixing cyanobacteria
Authors: ALAGESAN, S
GAUDANA, SB
KRISHNAKUMAR, S
WANGIKAR, PP
Keywords: Mixotrophic
Fed-Batch
Cyanothece
Feeding Strategy
Photolimitation
Issue Date: 2013
Publisher: ELSEVIER SCI LTD
Citation: BIORESOURCE TECHNOLOGY, 148228-233
Abstract: In the present study, fed-batch cultivation of Cyanothece sp. ATCC 51142, a known hydrogen producer, was optimized for maximizing biomass production. Decline in growth of this organism in dense cultures was attributed to increased substrate consumption for maintenance and respiration, and photolimitation due to self shading. A model incorporating these aspects was developed, and by using control vector parameterization (CVP), substrate feeding recipe was optimized to achieve 12-fold higher biomass concentration. The optimization results were verified experimentally on shake flask and bioreactor. The latter resulted in greater exponential growth rate possibly by overcoming photolimitation by simulating flashing light effect. Such a strategy can be readily applied for mixotrophic cultivation of cyanobacterial cultures in the first stage followed by photoautotrophic growth at the production stage. (C) 2013 Elsevier Ltd. All rights reserved.
URI: http://dx.doi.org/10.1016/j.biortech.2013.08.144
http://dspace.library.iitb.ac.in/jspui/handle/100/16107
ISSN: 0960-8524
1873-2976
Appears in Collections:Article

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.