Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/xmlui/handle/100/15383
Title: Cu(II) complexes of glyco-imino-aromatic conjugates in DNA binding, plasmid cleavage and cell cytotoxicity
Authors: KUMAR, A
MITRA, A
AJAY, AK
BHAT, MK
RAO, CP
Keywords: Glyco-Metal-Complexes
Dna Cleavage
Cytotoxicity
Apoptosis
Issue Date: 2012
Publisher: INDIAN ACAD SCIENCES
Citation: JOURNAL OF CHEMICAL SCIENCES, 124(6)1217-1228
Abstract: Binding of metal complexes of C2-glucosyl conjugates with DNA has been established by absorption and fluorescence studies. Conformational changes occurred in DNA upon binding have been studied by circular dichroism. All these studies are suggestive that the metal complexes bind to DNA through intercalation. Binding of di-nuclear copper complex 5 was found to be stronger when compared to the other complexes studied. Copper complexes were found to cleave the plasmid DNA in the absence of oxidizing or reducing agent, whereas, zinc complexes do not cleave. Metal complexes have shown toxicity to the HeLa and MCF-7 cell lines. Morphological studies, western blot and FACS analysis are suggestive of apoptotic cell death induced by the metal complexes. Di-nuclear copper complexes were found to be better as compared to the mononuclear ones in binding, plasmid cleavage and also in causing more cell death.
URI: http://dx.doi.org/10.1007/s12039-012-0319-0
http://dspace.library.iitb.ac.in/jspui/handle/100/15383
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