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|Title:||Type II phosphatidylinositol 4-kinase beta is an integral signaling component of early T cell activation mechanisms|
T Cell Activation
|Publisher:||ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER|
|Abstract:||The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (Ptdlns 4-kinases) are implicated as key components in these early signaling events. The role of type II PtdIns 4-kinase beta in CD3 receptor signaling was investigated with the help of short hairpin RNA sequences. Cross-linking of CD3 receptors on Jurkat T Cells with monoclonal antibodies showed an early increase in type II PtdIns 4-kinase activity and co-localization of type II PtdIns 4-kinase beta with CD3 zeta. Transfection of Jurkat T Cells with shRNAs inhibited CD3 receptor mediated type II PtdIns 4-kinase activation with a concomitant reduction in intra cellular calcium release, suggesting a role for type II PtdIns 4-kinase beta in CD3 receptor signal transduction. Knock-down of type II PtdIns 4-kinase beta with shRNAs also correlated with a decrease in PtdIns 4-kinase activity in cytoskeleton fractions and reduced adhesion to matrigel surfaces. These results indicate that type II Ptdlns 4-kinase beta is a key component in early T cell activation signaling cascades. Published by Elsevier Masson SAS.|
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