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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/100/14040

Title: Effect of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on surfactant monolayers
Authors: SHAH, AR
BANERJEE, R
Keywords: RESPIRATORY-DISTRESS-SYNDROME
ACUTE LUNG INJURY
PULMONARY SURFACTANT
OLEIC-ACID
N-ACETYLCYSTEINE
FILM FORMATION
VITAMIN-E
IN-VITRO
MODEL
FLUORESCENCE
Issue Date: 2011
Publisher: ELSEVIER SCIENCE BV
Citation: COLLOIDS AND SURFACES B-BIOINTERFACES,85(2)116-124
Abstract: In the present study, the effects of an amphiphilic polymer, D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) on model surfactant monolayers dipalmitoylphosphatidylcholine (DPPC), a binary mixture of DPPC with palmitoyloleoyl phosphatidylglycerol (DPPC-POPG) 9:1 (w/w) and binary mixture of DPPC and oleic acid (DPPC-OA) were evaluated. The ability of TPGS to act as an antioxidant adjuvant for pulmonary surfactants was also evaluated. Compression isotherms of surfactant monolayers at 37 degrees C in a Langmuir-Blodgett trough showed that DPPC and DPPC:TPGS mixed monolayers (1:0.25-1:1, w/w) exhibited low minimum surface tensions (MST) of 1-2 mN/m. Similarly [DPPC:POPG (9:1, w/w)]:TPGS mixed films of 1:0.25-1:1 weight ratios reached 1-2 mN/m MST. DPPC:POPG:TPGS liposomes adsorbed to surface tensions of 29-31 mN/m within 1 s. While monolayers of DPPC:OA (1:1, w/w) reached high MST of similar to 11 mN/m, DPPC:OA:TPGS (1:1:0.25, w/w) film reached near zero MST suggesting that low concentrations of TPGS reverses the effect of OA on DPPC monolayer. Capillary surfactometer studies showed DPPC:TPGS and [DPPC:POPG (9:1, w/w)]:TPGS liposomes maintained 84-95% airway patency. Fluorescence spectroscopy of Laurdan loaded DPPC:TPGS and DPPC:POPG:TPGS liposomes revealed no segregation of lipid domains in the lipid bilayer. Addition of TPGS to soybean liposome significantly reduced thiobarbituric acid reactive substance (TBARS) by 29-39% confirming its antioxidant nature. The results suggest a potential use of TPGS as an adjuvant to improve the surfactant activity as well as act as an antioxidant by scavenging free radicals. (C) 2011 Elsevier B.V. All rights reserved.
URI: http://dx.doi.org/10.1016/j.colsurfb.2011.01.021
http://dspace.library.iitb.ac.in/jspui/handle/100/14040
ISSN: 0927-7765
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