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|Title:||Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength|
|Keywords:||Field Absorption Sensor|
|Publisher:||ELSEVIER ADVANCED TECHNOLOGY|
|Citation:||BIOSENSORS & BIOELECTRONICS,26(7)3367-3370|
|Abstract:||A novel label-free technique for the detection of pathogens based on evanescent wave absorbance (EWA) changes at 280 nm from a U-bent optical fiber sensor is demonstrated. Bending a decladded fiber into a U-shaped structure enhances the penetration depth of evanescent waves and hence sensitivity of the probe. We show that the enhanced EWA response from such U-bent probes, caused by the inherent optical absorbance properties of bacterial cells or biomolecules specifically bound to the sensor surface, can be exploited for the detection of pathogens. A portable optical set-up with a UV light emitting diode, a spectrometer and U-bent fiber optic probe of 200 core diameter, 0.75 mm bend radius and effective probe length of 1 cm demonstrated an ability to detect less than 1000 cfu/ml. (C) 2011 Published by Elsevier B.V.|
|Appears in Collections:||Article|
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