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Please use this identifier to cite or link to this item: http://dspace.library.iitb.ac.in/jspui/handle/100/14028

Title: Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength
Authors: BHARADWAJ, R
SAI, VVR
THAKARE, K
DHAWANGALE, A
KUNDU, T
TITUS, S
VERMA, PK
MUKHERJI, S
Keywords: FIELD ABSORPTION SENSOR
U-SHAPED PROBE
GUIDE SENSOR
SENSITIVITY
BACTERIA
O157-H7
CELLS
Issue Date: 2011
Publisher: ELSEVIER ADVANCED TECHNOLOGY
Citation: BIOSENSORS & BIOELECTRONICS,26(7)3367-3370
Abstract: A novel label-free technique for the detection of pathogens based on evanescent wave absorbance (EWA) changes at 280 nm from a U-bent optical fiber sensor is demonstrated. Bending a decladded fiber into a U-shaped structure enhances the penetration depth of evanescent waves and hence sensitivity of the probe. We show that the enhanced EWA response from such U-bent probes, caused by the inherent optical absorbance properties of bacterial cells or biomolecules specifically bound to the sensor surface, can be exploited for the detection of pathogens. A portable optical set-up with a UV light emitting diode, a spectrometer and U-bent fiber optic probe of 200 core diameter, 0.75 mm bend radius and effective probe length of 1 cm demonstrated an ability to detect less than 1000 cfu/ml. (C) 2011 Published by Elsevier B.V.
URI: http://dx.doi.org/10.1016/j.bios.2010.12.014
http://dspace.library.iitb.ac.in/jspui/handle/100/14028
ISSN: 0956-5663
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